Testosterone testing generally requires blood tests, including total testosterone, free testosterone, and other indicators. It is recommended to conduct the test between 8-10 am in the morning.

1. Preparation before testing
Avoid vigorous exercise and alcohol consumption 24 hours before testing, and maintain an empty stomach for 8-12 hours. Some medications, such as glucocorticoids, may interfere with the results, so it is necessary to inform the doctor of the medication history in advance. It is recommended to wear loose clothing for easy blood collection, and sit quietly for 15 minutes before testing to reduce stress hormone fluctuations.
2. Basic indicator testing
Basic testing includes total testosterone measurement, with a reference range of 2.8-8.8ng/ml for normal adult males and 0.1-0.75ng/ml for females. If necessary, additional testing of sex hormone binding globulin and albumin is required to calculate the free testosterone index. Some laboratories can provide salivary testosterone testing as a supplement.
3. Dynamic functional assessment [SEP]: For individuals suspected of hypogonadism, a chorionic gonadotropin stimulation test can be performed. The standard procedure is to recheck testosterone 48 hours after intramuscular injection of HCG2000IU. If the increase is less than 2ng/ml, it indicates dysfunction of testicular interstitial cells. This experiment needs to be conducted under the supervision of a specialist physician.
4. Joint Inspection of Relevant Indicators

It is recommended to simultaneously detect luteinizing hormone and follicle stimulating hormone to evaluate the function of the hypothalamic pituitary gonadal axis. The detection of five thyroid functions and cortisol circadian rhythm can help identify secondary causes. Bone density examination can evaluate the impact of long-term low testosterone on bones.
5. interpretation of Results
requires comprehensive judgment based on clinical manifestations, and a single abnormal result should be reviewed every 4-6 weeks. For men over 40 years old, screening is recommended every year. Patients with chronic diseases such as diabetes can increase the monitoring frequency. The test report should include age stratification reference values, and abnormal results should be referred to the endocrinology department. Maintaining a regular daily routine and moderate exercise can help maintain testosterone levels, while zinc and vitamin D deficiencies can be improved through dietary supplementation. Avoiding long-term exposure to environmental endocrine disruptors can significantly increase testosterone levels in obese patients by reducing their weight by 5-10%. If symptoms such as decreased sexual function and reduced body hair occur, seek medical attention promptly.

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