Rabies can be preliminarily diagnosed based on clinical symptoms combined with exposure history, and diagnosis relies on laboratory testing. The main examination methods include virus antigen testing, nucleic acid testing, brain tissue pathological examination, etc.

1. Exposure history assessment
Review whether animals such as dogs, cats, bats, etc. have bitten or scratched recently, especially unvaccinated animals. Individuals who fail to promptly treat their wounds or receive vaccinations after exposure are at a higher risk. If there is a suspected history of exposure and abnormal symptoms appear, seek medical attention immediately.
2. Early symptom observation
In the early stages, non-specific symptoms such as fever, headache, abnormal wound pain or itching may occur. Some patients may experience neurological symptoms such as anxiety and insomnia. At this stage, symptoms are easily confused with other infections and need to be comprehensively judged based on exposure history.
3. Recognition of Excitatory Symptoms
Typical manifestations include fear of water, wind, and spasms in the throat muscles, which may be accompanied by mental symptoms such as mania and hallucinations. The patient has increased saliva secretion and difficulty swallowing, which is highly contagious. This period is an important basis for clinical diagnosis.

4. Laboratory testing
detects viral antigens in corneal or skin biopsy samples using fluorescent antibody method, or detects viral RNA in saliva and cerebrospinal fluid using RT-PCR technology. Diagnosis after death requires the discovery of endosomes through pathological examination of brain tissue.
5. Differential diagnosis
requires the exclusion of diseases such as tetanus and viral encephalitis. Tetanus is characterized by muscle rigidity throughout the body but no symptoms of hydrophobia, and encephalitis often has no clear history of animal contact. The final diagnosis needs to be completed by a professional medical institution. After suspected exposure to rabies, the wound should be immediately rinsed alternately with soapy water and running water for 15 minutes, and disinfected with iodine. All suspected exposures must be promptly assessed at the rabies vaccination clinic and vaccinated and immunoglobulin administered according to exposure levels. Avoid contact with wild animals in daily life, and regularly vaccinate domestic pets against rabies. If there are suspected symptoms, immediately isolate and contact the CDC, and do not delay treatment.

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